Category: news
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WHO tuberculosis mutation catalogue released
Last Friday 25 June 2021, the World Health Organization released their first report containing a catalogue of mutations in Mycobacterium tuberculosis and their association with drug resistance. The CRyPTIC project shared its large and comprehensive dataset, as well as providing its genetic processing pipeline, to the group compiling that data and producing the report. We are very pleased that…
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Internal project seminars in June 2021
The analysis group will be presenting five consecutive seminars starting on Wed 9 June at 1500 BST (1400 GMT). Each seminar will focus on one of the primary publications to come from the CRyPTIC project. In the first seminar we will begin by describing the Release One dataset The CRyPTIC Release One dataset: how it…
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New preprint: Deciphering bedaquiline and clofazimine resistance in tuberculosis
Stefan Niemann, a CRyPTIC partner, led this combined experimental and modelling study looking at how resistance is conferred to bedaquline and clofazimine in Mycobacterial tuberculosis. The CRyPTIC project provided 14,151 clinical isolates, each with minimum inhibitory concentrations (MICs) to bedaquiline and clofazimine. This dataset was used to pinpoint genetic variation in Rv0678, a transcription regulator of a…
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New preprint: Establishing epidemiological cutoffs for the CRyPTIC UKMYC microdilution plates.
The CRyPTIC project has measured the minimum inhibitory concentrations (MICs) of 20,637 isolates to 13 different anti-TB drugs using a bespoke 96-well broth microdilution plate. These samples were collected by 14 partner laboratories based in 11 countries and span five continents. In this preprint, we use this large dataset to determine epidemiological cutoffs (ECOFF or ECV)…
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BashTheBug passes 4 million classifications
A few days ago our BashTheBug volunteers passed the 4 million classifications mark! The work they are doing is key to improving the accuracy with which our 96-well microtitre plates are read in the CRyPTIC project. For more information see the BashTheBug blog.
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New paper: Estimating genetic adaption in 10,000 M. tuberculosis genomes
Daniel Wilson, one of the CRyPTIC partners, has recently published a paper where he introduces a new approach, genomegaMap, which estimates the ratio of non-synonymous to synonymous mutations (usually referred to as dN/dS) at sites across the genome. This ratio can provide important clues to which parts of the genome are under selection pressure, which can lead…
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CRyPTIC inoculates its 15,000th microtitre plate
Sometime in November 2019, the 15,000th 96-well microtitre plate was inoculated with M. tuberculosis. Since each plate contains 13 different antibiotics used to treat tuberculosis this is a large and comprehensive antibiotic susceptibility dataset. The vast majority of samples also have their whole genome sequenced to enable us to associate genetic variants with resistance (or susceptibility).
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Fifth Annual CRyPTIC Meeting in Hyderabad
The fifth CRyPTIC annual meeting took place in Hyderabad on Sat 26 and Sun 27 Oct 2019. Consortium partners were updated on the various analysis projects and a General Assembly was held to discuss the progress and direction of the project.
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Groundbreaking publication
Groundbreaking publication in NEJM on using DNA sequencing for drug resistance prediction in TB. For the first time, our understanding of the genetics is accurate enough to confirm that the 4 first-line drugs (isoniazid, rifampicin, ethambutol and pyrazinamide) will be effective. See NEJM editorial here and a number of press releases from European Bioinformatics Institute, Oxford Biomedical Research Centre, Genomics…
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BBC video shining light on the deadliest of infectious diseases: TB
Watch this nice BBC video featuring CRyPTIC’s member Tim Walker talking about TB diagnosis: http://www.bbc.co.uk/guides/zpnp6yc